SEROLOGICAL AND IMMUNOHISTOCHEMICAL DIAGNOSIS OF TOXOPLASMOSIS AMONG ABORTED WOMEN IN SORAN DISTRICT, KURDISTAN- IRAQ
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Toxoplasma gondii, Seroprevalence, Chemiluminescence Immunoassay, Immunohistochemistry, Risk factors, Aborted women
Abstract
Toxoplasma gondii is a zoonotic intracellular protozoan parasite with a cosmopolitan distribution that causes severe health complications known as toxoplasmosis, especially among pregnant women and immunocompromised people. This investigation aimed to assess the prevalence of T. gondii in serum and placental tissue of aborted women in Soran city, Iraq, and its correlation with various socio-demographic characteristics, as well as associated risk factors. Blood specimens (250) from spontaneously aborted females were verified for anti-Toxoplasma IgG and IgM antibodies by Chemiluminescent Immunoassay (CLIA). The immunohistochemistry (IHC) technique was also employed to examine placental samples for the confirmation of the parasite. The CLIA assay explored that 19.6% of women who underwent abortions showed positive anti-Toxoplasma IgG antibodies, indicating a past or chronic infection, while 1.2% displayed positive anti-Toxoplasma IgM, suggesting an acute or recent infection. All placental tissue from CLIA-seropositive women showed a positive reaction by IHC, indicating the presence of the parasite antigen. This study found that the highest rate of abortion occurred among women aged 26-35 years, at 22.9% (30/131). Most pregnancies ended in abortion during the first trimester. The consumption of undercooked meat was most significantly affecting the seroprevalence of anti-Toxoplasma IgG antibodies (P value = 0.008). In comparison, the factor influencing anti-Toxoplasma IgM antibodies was the level of education, with a P value of 0.031. This research revealed a high rate of toxoplasmosis among women who experienced pregnancy loss. It is advisable to raise awareness about risk factors and to test pregnant women during their gynecological care.
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References
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